RBS

Part:BBa_K2656010:Design

Designed by: Adrián Requena Gutiérrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-20)


Weak Ribosome Binding Site B0032


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has been designed adding to the RBS sequence: GCGCCGTCTCGCTCGTACT upstream and tactagAATGTGAGCGAGACGGCGC downstream. These sequences allow the assembly of the part into BBa_P10500 with a Golden Gate reaction using BsmbI endonuclease. We have added the equivalent of the BioBrick scar to the RBS sequence because we have noted that without it, the transcriptional units with this RBS had not expression.

Source

The original sequence has been obtained from the Registry of Standard Biological Parts and it has been synthetized by IDT.


References